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Using Spectrophotometric Methods, Evaluation of Antibiotic Drug with Different Metals Interaction study, an In Vitro Analysis

Mohammad Razaul Karim, Md. Shahidul Islam


The present study describes of interaction studies of cephradine with essential metals. Cephradine is included among the 1st generation of cephalosporin drug class which is active against a wide range of gram positive and gram negative bacteria. By this study, it is observed that cephradine with essential and trace elements of metals present in the body. Cephradine has been interacted with Mg, Mn, Zn and Fe metal salts in vitro. All the reaction conditions were simulated to natural environments.The studies were carried out in buffer at PH 7.4 in variable ratios of drug and metal both at room temperature conditions. It has been observed that almost major proportion of drug is complexes with metals.There is an effect of PH on drug metal complexation. At pH 7.4, it is investigated that cephradine forms complex and interacts with different essential metals. So, the stability constant of these complexes were determined in order to evaluate their possible in vivo implications. By this interaction antibiotic with different essential metals, the activities of antibiotic will be decreased. The ultraviolet spectrophotoetric studies of these complexes were carried out and compared.

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